United States · Admission test

DAT subjects and topics, section by section

Six subjects make up the DAT: biology, general chemistry, organic chemistry, perceptual ability, reading comprehension and math. ADA’s 2026 Candidate Guide lists the topics for each one, and it says you don’t need advanced biology or physics. The test uses both US and metric units.

What subjects are on the DAT?

SectionWhat ADA lists
Biology (40 questions)Cell and molecular biology, diversity of life, structure and function of systems, genetics, evolution and ecology
General chemistry (30)Stoichiometry, gases, liquids and solids, solutions, acids and bases, equilibria, thermodynamics, kinetics, redox, atomic and molecular structure, periodic properties, nuclear reactions, lab
Organic chemistry (30)Mechanisms, chemical synthesis, acid-base chemistry, chemical and physical properties of molecules, structural evaluation
Perceptual Ability (90)Apertures, view recognition, angle discrimination, paper folding, cube counting, 3D form development
Reading Comprehension (50)Three passages on science topics; you don’t need to know the topic beforehand
Quantitative Reasoning (40)Algebra, data analysis and sufficiency, quantitative comparison, probability and statistics, word problems

Biology topics in detail

  • Cell and molecular biology: metabolism (including photosynthesis and enzymes), membrane transport and signaling, thermodynamics, mitosis and meiosis, cell structure, experimental cell biology and biomolecules.
  • Diversity of life: viruses, archaebacteria, eubacteria, fungi, protists, plants and animals.
  • Structure and function of systems: integumentary, skeletal, muscular, circulatory, lymphatic and immune, digestive, respiratory, urinary, nervous and sensory, endocrine and reproductive.
  • Genetics: molecular, human, classical and chromosomal genetics, genetic technology, development, genomics, gene expression and epigenetics.
  • Evolution and ecology: natural selection, population genetics and speciation, animal behavior, and population, community and ecosystem ecology.

Chemistry topics in detail

  • General chemistry includes gas laws (Dalton, Boyle, Charles and the ideal gas law), colligative properties, pH and Brønsted-Lowry reactions, Le Chatelier’s principle, Hess’s law, rate laws and half-life, oxidation numbers and electrochemistry, and lab error analysis and safety. A periodic table is on screen for this part.
  • Organic chemistry, under the specifications in place since April 2026: curved-arrow mechanisms and reaction coordinate diagrams; one-step, two-step and multi-step synthesis (SN1, SN2, E1, E2 and reactions of alkenes, alcohols, ethers, aromatics, carbonyls and more); ranking acidity and basicity; polarity, solubility and boiling points; chromatography, extraction, recrystallization and distillation; 1H NMR, 13C NMR and IR spectroscopy; and nomenclature, stereochemistry and bonding.

Perceptual ability, reading and math

Perceptual Ability is about picturing shapes in space, with no science or math. Reading Comprehension tests how well you read and analyze science writing, not what you already know. Quantitative Reasoning is first-year college math with a basic calculator. Each has its own page with more detail.

Sources: ADA and 1 more. Checked October 2026.

Rules and fees can change, so check the official site before your test.